An FRT cassette separates the αTub84B promoter from sequence encoding full-length wts that is tagged at the N-terminal end with Citrine and at the C-terminal end with mTFP1. mTFP1 and Citrine can act as a fluorescence resonance energy transfer (FRET) pair (donor and acceptor respectively), and changes in the quenching ratio (acceptor channel emissions/donor channel emissions) can be used to monitor conformational changes in the wts protein in vivo. The stop cassette does not abolish transcription of the tagged wts protein, but reduces expression levels such that the transgene generally fails to rescue a wts loss of function mutant to adulthood.