FB2024_03 , released June 25, 2024
Recombinant Construct: P{BEAF-32B-mRFP1-NLS.ΔM-NC}
Open Close
General Information
Symbol
P{BEAF-32B-mRFP1-NLS.ΔM-NC}
FlyBase ID
FBtp0158494
Feature type
transgenic_transposable_element
Component Allele(s)
BEAF-32ΔM-NC.B.mRFP1.Tag:NLS(SV40-largeT)
Size
Expression Data
Associated insertion(s)
0 available
Molecular map
Description and Uses
Construct components
Component allele BEAF-32ΔM-NC.B.mRFP1.Tag:NLS(SV40-largeT)
Transgenic product class
inframe_deletion
(Avva and Hart, 2016)
Component
Use(s)
Regulatory region(s)
BEAF-32
Encoded product / tool
BEAF-32
Tagged with
mRFP1
Tag:NLS(SV40-largeT)
Description

A fragment that encompasses the BEAF-32 transcription unit, with upstream sequence extending to -900bp relative to the ATG codon of the BEAF-32 A isoform. The C-terminal end of the BEAF-32 coding sequence (sequence common to both isoforms) is tagged with mRFP1 and with Tag:NLS(SV40-largeT). Sequence extending from approximately -30 to +140 relative to the ATG codon of the A isoform has been deleted. Sequence corresponding to amino acid residues 157-198 of the B isoform has been deleted. This results in a transgene which expresses only the mutant B isoform (tagged with mRFP1).

(Avva and Hart, 2016)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P{BEAF-32B-mRFP1-NLS.ΔM-NC}
(FlyBase, 1992-)
ΔM-NC-mRFP-NLS
(Avva and Hart, 2016)
Secondary FlyBase IDs
    References (2)