FB2024_03 , released June 25, 2024
Recombinant Construct: P{UAS-pH-CaspGFP-2A-pHlorina}
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General Information
Symbol
P{UAS-pH-CaspGFP-2A-pHlorina}
FlyBase ID
FBtp0150860
Feature type
transgenic_transposable_element
Component Allele(s)
Avic\GFPpH-CaspGFP.UAS
Disc\RFPpHlorina.UAS
Size
Expression Data
Associated insertion(s)
0 available
Molecular map
Description and Uses
Construct components
Component allele Avic\GFPpH-CaspGFP.UAS
Transgenic product class
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Avic\GFP
Description

UASt regulatory sequences drive expression of 'CharON' (Caspase and pH Activated Reporter, Fluorescence ON), a reporter that is designed for tracking emerging apoptotic cells and their efferocytic clearance by phagocytes. The CharON reporter consists of two open reading frames (ORFs) separated by a P2A peptide (to ensure separate translation of the two ORFs). The first ORF corresponds to pH-CaspGFP (GC3ai with pH-stabilizing mutation Q204H), a green a fluorescent sensor of caspase activity, the second ORF corresponds to pHlorina (a red fluorescent protein that shows increasing fluorescent with decreasing pH).

(Raymond et al., 2022)
Component allele Disc\RFPpHlorina.UAS
Transgenic product class
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
pHlorina
Description

UASt regulatory sequences drive expression of 'CharON' (Caspase and pH Activated Reporter, Fluorescence ON), a reporter that is designed for tracking emerging apoptotic cells and their efferocytic clearance by phagocytes. The CharON reporter consists of two open reading frames (ORFs) separated by a P2A peptide (to ensure separate translation of the two ORFs). The first ORF corresponds to pH-CaspGFP (GC3ai with pH-stabilizing mutation Q204H), a green a fluorescent sensor of caspase activity, the second ORF corresponds to pHlorina (a red fluorescent protein that shows increasing fluorescent with decreasing pH).

(Raymond et al., 2022)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P{UAS-pH-CaspGFP-2A-pHlorina}
(FlyBase, 1992-)
uas-charon
(Raymond et al., 2022)
Secondary FlyBase IDs
    References (2)