UASt regulatory sequences drive expression of the Hsap\PRPS1L1 open reading frame. The endogenous stop codon is present, thus even though three copies of the Tag:HA tag are present downstream of the ORF, they are not expected to form part of the translated protein. The ORF has been mutated to carry p.G61D (c.182G>A), a variant associated with Autism Spectrum disorder (PMID: 25363768).