UASt regulatory sequences drive expression of the Hsap\DPYSL3 open reading frame, tagged at the C-terminal end with three copies of Tag:HA. The ORF (originally amplified from the ccsbBroadEn_06119 clone) has been mutated to carry p.V139I (c.415G>A), a variant associated with Autism Spectrum disorder (PMID:25363768).