UASt regulatory sequences drive expression of the Hsap\SLC23A1 open reading frame. The endogenous stop codon is present, thus even though three copies of the Tag:HA tag are present downstream of the ORF, they are not expected to form part of the translated protein. The ORF (originally amplified from the IOH26574 clone) has been mutated to carry p.L465M (c.1393C>A), a variant associated with Autism Spectrum disorder (PMID:25363768).