UASt regulatory sequences drive expression of the Hsap\CLCNKB open reading frame, tagged at the C-terminal end with three copies of Tag:HA. The ORF (originally amplified from the ccsbBroadEn_10737 clone) has been mutated to carry p.M176I (c.528G>C), a variant associated with Autism Spectrum disorder (PMID:25363768).