FB2024_03 , released June 25, 2024
Recombinant Construct: P{Cre80Tom-GFP}
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General Information
Symbol
P{Cre80Tom-GFP}
FlyBase ID
FBtp0143269
Feature type
transgenic_transposable_element
Component Allele(s)
Mmus\Cd8a10xUAS.GFP
Scer\GAL80Act5C.loxP.Tom
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele Scer\GAL80Act5C.loxP.Tom
Transgenic product class
Component
Use(s)
Regulatory region(s)
Act5C
Encoded product / tool
GAL80
Also carries
loxP
Description

An Act5C promoter is fused upstream of a loxP cassette containing GAL80. The loxP cassette is followed by the tdTomato coding region that is tagged with Tag:Myr(Unk). In the absence of P1cre recombinase, GAL80 is expressed under the control of the Act5C promoter. The Tag:Myr(Unk)-tagged tdTomato reporter is only expressed after excision of the loxP cassette using P1cre recombinase.

(Luan et al., 2020)
Component allele Mmus\Cd8a10xUAS.GFP
Transgenic product class
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Mmus\Cd8a
Tagged with
GFP
Description

Ten copies of the UAS site followed by an Hsp70 basal promoter are fused upstream of a Mmus\Cd8a-Avic\GFP fusion protein. An SV40 transcriptional terminator is present.

(Pfeiffer et al., 2010)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Cre80Tom-GFP
(Luan et al., 2020)
P{Cre80Tom-GFP}
(FlyBase, 1996-)
Secondary FlyBase IDs
    References (2)