FB2024_03 , released June 25, 2024
Recombinant Construct: PBac{tup-CD4-tdGFP.AME-R}
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General Information
Symbol
PBac{tup-CD4-tdGFP.AME-R}
FlyBase ID
FBtp0142467
Feature type
transgenic_transposable_element
Component Allele(s)
Avic\GFPtdGFP.tup.AME-R.Tag:M(hCD4)
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele Avic\GFPtdGFP.tup.AME-R.Tag:M(hCD4)
Transgenic product class
Component
Use(s)
Encoded product / tool
EGFP
GFP
Tagged with
Tag:M(hCD4)
Tag:SS(Akh)
Description

A 452bp fragment from the tup upstream region (coordinates 2L:18900307..18899855 , release 6 genome) is fused upstream of a membrane-targeted fusion protein containing the Tag:SS(Akh) signal peptide, the Hsap\CD4 transmembrane domain, a tandem fluorescent dimer composed of EGFP and GFP and an endoplasmic reticulum export signal from the Hsap\KCNJ2 gene (generated using vector pDEST-HemmarG, Addgene:31221 ).

(Bataillé et al., 2020)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
AMER-cd4-tdGFP
(Bataillé et al., 2020)
PBac{tup-CD4-tdGFP.AME-R}
(FlyBase, 1992-)
Secondary FlyBase IDs
    References (2)