UAS regulatory sequences drive expression of two different sgRNAs, both of which target common coding exons within the 5' half of the mip130 gene without overlapping the start codon; this is designed to result in an indel (small insertion or deletion mutation) of the target gene in the presence of a source of Cas9. Designed for conditional CRISPR mutagenesis; a GAL4 driver can be used to express the sgRNAs and a UAS-Cas9 transgene in a tissue-specific manner, inducing mutagenesis only in the GAL4-expressing cells. In addition, the sgRNA cassette is flanked by a pair of incompatible FRT sites (FRT2 and FRT5), which allows for future in vivo exchange of sequences upstream or downstream of the sgRNAs.
UAS regulatory sequences drive expression of two different sgRNAs, at least one of which targets exons of Rbcn-3B; this may result in an indel (small insertion or deletion mutation) of the target gene in the presence of a source of Cas9. Designed for conditional CRISPR mutagenesis; a GAL4 driver can be used to express the sgRNAs and a UAS-Cas9 transgene in a tissue-specific manner, inducing mutagenesis only in the GAL4-expressing cells.