FB2024_03 , released June 25, 2024
Recombinant Construct: M{UAS-pros.ORF-VN}
Open Close
General Information
Symbol
M{UAS-pros.ORF-VN}
FlyBase ID
FBtp0134808
Feature type
transgenic_transposable_element
Component Allele(s)
prosUAS.ORF-VN
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component
Use(s)
Also carries
loxP
Marker(s)
w+mC
Component allele prosUAS.ORF-VN
Transgenic product class
wild_type
(Bischof et al., 2018)
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
pros
Tagged with
N-m9Venus
Also carries
FRT2
FRT5
Description

UASt regulatory sequences drive expression of the pros open reading frame, tagged at the C-terminal end with the N-m9Venus split fluorescent protein fragment. Designed for use in a bimolecular fluorescence complementation (BiFC) assay. The pros ORF is flanked by a pair of incompatible FRT sites (FRT5 and FRT2), which allows for future in vivo exchange of either the promoter or the C-terminal tag sequence. A linker of 24 residues separates the FRT2 and N-m9Venus sequences.

(Bischof et al., 2018)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (1)
Stocks containing insertion(s) of this recombinant construct
FlyORF
F004799
M{UAS-pros.ORF-VN}ZH-86Fb
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
M{UAS-pros.ORF-VN}
(FlyBase, 1996-)
UAS-pros ORF
(Katti et al., 2022)
Secondary FlyBase IDs
    References (3)