FB2024_03 , released June 25, 2024
Recombinant Construct: P{UAS-MApHS}
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General Information
Symbol
P{UAS-MApHS}
FlyBase ID
FBtp0128025
Feature type
transgenic_transposable_element
Component Allele(s)
Hsap\CD4MApHS.UAS
Also Known As
UAS-MApHS
Size
Expression Data
Associated insertion(s)
0 available
Molecular map
Description and Uses
Construct components
Component allele Hsap\CD4MApHS.UAS
Transgenic product class
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
pHluorinE
tdTomato
Tagged with
Tag:M(hCD4)
Description

UAS regulatory sequences drive expression of a membrane-associated pH sensor ('MApHS') designed to analyze phagosome maturation. This consists of the pH-sensitive green fluorescent protein pHluorinE (extracellular), a transmembrane domain (Tag:M(hCD4)) and the tdTomato red fluorescent protein (cytoplasmic). Since pHluorinE loses fluorescence as the pH is lowered (losing fluorescence below pH 6.0), the sensor is expected to lose green fluorescence but retain red fluorescence upon acidification of phagosomes.

(Han et al., 2014)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P{UAS-MApHS}
(FlyBase, 1992-)
UAS-MApHS
(Ji et al., 2022, Allen et al., 2021, Han et al., 2014)
Secondary FlyBase IDs
    References (4)