FB2024_03 , released June 25, 2024
Recombinant Construct: PBac{UAS-Vt-TSmod-Vt}
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General Information
Symbol
PBac{UAS-Vt-TSmod-Vt}
FlyBase ID
FBtp0126821
Feature type
transgenic_transposable_element
Component Allele(s)
Ggal\VincUAS.Vt-TSmod-Vt
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele Ggal\VincUAS.Vt-TSmod-Vt
Transgenic product class
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Ggal\Vinc
Description

UAS regulatory sequences drive expression of a sensor that monitors myosin activity under physiological conditions. The sensor consists of two Ggal\Vinc tail domains, each of which can bind filamentous actin, with a fluorescence resonance energy transfer (FRET)-based tension-sensing module inserted between them. The tension-sensing module comprises two fluorescent proteins - mTFP1 (FRET donor) and Venus (FRET acceptor) - separated from each other by a stretchable domain from spider silk (PMID:20613844). If actin filaments are pulled together due to myosin activity, the sensor will produce high a fluorescence resonance energy transfer (FRET) signal, providing an indirect readout of myosin activity.

(Tsankova et al., 2017)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
PBac{UAS-Vt-TSmod-Vt}
(FlyBase, 1992-)
TSmod-Vt
(Roubinet et al., 2017)
Vt-TS-Vt
(Tsankova et al., 2017)
Secondary FlyBase IDs
    References (3)