QUAS regulatory sequences (followed by a minimal Hsp70 promoter) drive expression of the tdTomato fluorescent protein, tagged at the N-terminal end with Tag:MyrPalm(MGCCFSKT) (which directs myristoylation and palmitoylation) and at the C-terminal end with 3 copies of the Tag:HA epitope.
Ten copies of the UAS site followed by an Hsp70 basal promoter are fused upstream of EGFP that has been optimised for Drosophila codon usage. The EGFP coding sequence is tagged at the N-terminal end with a myristoylation signal (Tag:Myr(Src64B)). A 67bp intron derived from Mhc has been inserted in the 5' UTR. An SV40 transcriptional terminator is present.