FlyBase Recombinant Construct Report: P{lexAop-nSyb-spGFP1-10}

General Information

Symbol

P{lexAop-nSyb-spGFP1-10}

FlyBase ID

FBtp0111033

Feature type

transgenic_transposable_element

Component Allele(s)

nSyb^{lexAop.spGFP1-10}

Also Known As

LexAop-nSyb-spGFP1-10, Aop-syb:spGFP1-10

Size

Expression Data

Associated insertion(s)

1 available

Molecular map

Description and Uses

Construct components

Component allele nSyb^{lexAop.spGFP1-10}

Transgenic product class

Component

Use(s)

Regulatory region(s)

lexAop

binary expression system - regulatory region

Encoded product / tool

nSyb

Tagged with

spGFP1-10

split fluorescent protein

Description

lexAop regulatory sequences drive expression of the spGFP1-10 split fluorescent protein fragment fused to the cytoplasmic domain of nSyb, thus targeting the protein to pre-synaptic sites. This construct is designed for use in a GRASP (GFP Reconstitution Across Synaptic Partners) assay, in which complementary split fluorescent protein fragments expressed on the extracellular membrane of different neurons can reconstitute a functional fluorescent protein at points of contact between the neurons.

(Frank et al., 2015)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

nSyb^{lexAop.spGFP1-10}

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

descendant(s)

Comments

Stocks (1)

Stocks containing insertion(s) of this recombinant construct

Bloomington

64315

w^*; P{lexAop-nSyb-spGFP1-10}2, P{UAS-CD4-spGFP11}2; MKRS/TM6B

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (11)

Reported As

Symbol Synonym

AOP.Syb:spGFP1-10

(Frank et al., 2015)

Aop-syb:spGFP1-10

(Frank et al., 2015, Macpherson et al., 2015)

LexAop-Syb::spGFP1-10

(Sancer et al., 2019)

LexAop-nSyb-spGFP1-10

(Qi et al., 2021)

LexAop-nSyb:spGFP1-10

(Liu et al., 2019)

LexAop-nsyb-spGFP_1-10

(Coates et al., 2017)

LexAop-syb-spGFP1-10

(Tenedini et al., 2019)

LexO-nsyb:GFP_1-10

(Patel et al., 2022)

P{lexAop-nSyb-spGFP1-10}

(FlyBase, 1992-)

lexAop-nSyb-spGFP1-10

(Sancer et al., 2019, Shaw et al., 2019)

lexO-nsyb:GFP^1-10

(Du et al., 2018)

Secondary FlyBase IDs

References (11)

Report Sections

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