Mi{Trojan-3XGAL80.0} represents a transgenic construct generated in vivo by using phiC31:int-mediated recombination to replace the attP cassette of a Mi{MIC} element insertion with an intron phase 0 'Trojan 3XGAL80' cassette from the pBS-KS-attB2-SA(0)-T2A-3XGAL80-Hsp70 plasmid. The Trojan 3XGAL80 sequence consists of a splice acceptor site followed by: the T2A peptide - GAL80 tagged with both Tag:FLAG and Tag:HA - a P2A peptide - GAL80 tagged with EGFP - a T2A peptide - an untagged copy of GAL80 - an Hsp70 transcription termination signal. Integration of the cassette into a Mi{MIC} insertion in a coding intron (with the same phase) of a native Drosophila gene of interest will result in the cassette behaving as a 'Trojan' exon: the splice acceptor site ensures that the T2A-GAL80-P2A-GAL80-T2A-GAL80 open reading frame is incorporated into the mRNA of the native Drosophila gene, while the T2A and P2A sequences promote the separate translation of each GAL80 open reading frame (the first T2A sequence should also result in the truncation of the native gene product). Thus GAL80 should be expressed under the control of the regulatory sequences of the native Drosophila gene of interest in the resulting fly line.