Mi{Trojan-lexA:QFAD.0} represents a transgenic construct generated in vivo by using phiC31:int-mediated recombination to replace the attP cassette of a Mi{MIC} element insertion with an intron phase 0 'Trojan lexA:QFAD ' cassette from the pBS-KS-attB2-SA(0)-T2A-lexA:QFAD-Hsp70 plasmid. The Trojan lexA:QFAD cassette consists of a splice acceptor site followed by the T2A peptide, sequence encoding a lexA::QF driver and an Hsp70 transcription termination signal. Integration of the cassette into a Mi{MIC} insertion in a coding intron (with the same phase) of a native Drosophila gene of interest will result in the cassette behaving as a 'Trojan' exon: the splice acceptor site ensures that the T2A-lexA::QF open reading frame is incorporated into the mRNA of the native Drosophila gene, while the T2A sequence truncates the native gene product and promotes the separate translation of the lexA::QF open reading frame. Thus the lexA::QF driver should be expressed under the control of the regulatory sequences of the native Drosophila gene of interest in the resulting fly line.
One of 3 essentially identical transgenic constructs (Mi{Trojan-lexA:QFAD.0}, Mi{Trojan-lexA:QFAD.1} and Mi{Trojan-lexA:QFAD.2}) that vary only in the phase of the Trojan lexA:QFAD cassette.