FB2024_03 , released June 25, 2024
Recombinant Construct: M{Act5C-Cas9.P.RFP-}
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General Information
Symbol
M{Act5C-Cas9.P.RFP-}
FlyBase ID
FBtp0098099
Feature type
transgenic_transposable_element
Component Allele(s)
Spyo\Cas9Act5C.cPa
Also Known As
Act5C-Cas9, Act-Cas9
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele Spyo\Cas9Act5C.cPa
Transgenic product class
Component
Use(s)
Regulatory region(s)
Act5C
Encoded product / tool
Cas9
Description

A 4.4 kb fragment of the upstream regulatory region of the Act5C gene drives expression of human codon optimized Cas9 endonuclease (cloned from Addgene plasmid 41815).

(Port and Bullock, 2014.1.10)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
M{Act5C-Cas9.P}
(Zhang et al., 2014)
descendant(s)
Comments

The w+mC and Disc\RFP3xP3.PB markers have been excised from M{Act5C-Cas9.P} using P1\cre recombinase, generating M{Act5C-Cas9.P.RFP-}.

(Zhang et al., 2014)
Stocks (4)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
Act-Cas9
(Ji et al., 2022, Poe et al., 2019)
Act5C-Cas9
(Kandul et al., 2021, Yin et al., 2021)
M(Act5C-Cas9)
(Zhang et al., 2014)
M{Act5C-Cas9.P.RFP-}
(FlyBase, 1996-)
Secondary FlyBase IDs
    References (7)