FB2024_03 , released June 25, 2024
Recombinant Construct: P{Burs-mCD8-EGFP-T2A-Gal4}
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General Information
Symbol
P{Burs-mCD8-EGFP-T2A-Gal4}
FlyBase ID
FBtp0093265
Feature type
transgenic_transposable_element
Component Allele(s)
Size
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Construct components
Component allele Mmus\Cd8aBurs.EGFP
Transgenic product class
Component
Use(s)
Regulatory region(s)
Encoded product / tool
Description

The Burs promoter is fused upstream of Mmus\Cd8a fused to EGFP, followed by the "T2A" peptide from Thosea asigna and the GAL4 coding region. The "T2A" peptide supports ribosomal skipping, allowing the downstream GAL4 protein to be translated independently of the membrane bound Mmus\Cd8a-EGFP fusion protein, resulting in a functional GAL4 driver expressed under the control of Burs regulatory sequences.

Component allele Scer\GAL4Burs.PD
Transgenic product class
Component
Use(s)
Regulatory region(s)
Description

The Burs promoter is fused upstream of Mmus\Cd8a fused to EGFP, followed by the "T2A" peptide from Thosea asigna and the GAL4 coding region. The "T2A" peptide supports ribosomal skipping, allowing the downstream GAL4 protein to be translated independently of the membrane bound Mmus\Cd8a-EGFP fusion protein, resulting in a functional GAL4 driver expressed under the control of Burs regulatory sequences.

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
P{Burs-mCD8-EGFP-T2A-Gal4}
Secondary FlyBase IDs
    References (2)