FB2024_03 , released June 25, 2024
Recombinant Construct: P{(FRT.Ee)S500(loxP.Gy)-w}
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General Information
Symbol
P{(FRT.Ee)S500(loxP.Gy)-w}
FlyBase ID
FBtp0087695
Feature type
transgenic_transposable_element
Component Allele(s)
w+mC.Scer\FRT-Ee.S-500.P1\loxP-su(Hw)BR
Size
Expression Data
Associated insertion(s)
0 available
Molecular map
Description and Uses
Description

Transgenic construct used to investigate the possible role of z protein in long-range interactions between enhancers and promoters. Contains an FRT cassette containing the w eye enhancer (-1180 to -1450bp relative to the transcription start site), a 500bp DNA spacer, a loxP cassette containing a truncated gypsy\su(Hw)BR insulator (contains only 5 su(Hw) binding sites), and the w+mC mini-gene.

(Kostyuchenko et al., 2009)
Construct components
Component
Use(s)
Also carries
FRT
loxP
Component allele w+mC.Scer\FRT-Ee.S-500.P1\loxP-su(Hw)BR
Transgenic product class
Component
Use(s)
Encoded product / tool
w
Description

The w eye enhancer (Ee), flanked by Scer\FRT sites, is inserted 500bp upstream of a P1\loxP-flanked gypsy\su(Hw)BR insulator followed by the w+mC mini-gene.

(Kostyuchenko et al., 2009)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
(Ee)S500(Gy)W
(Kostyuchenko et al., 2009)
P{(FRT.Ee)S500(loxP.Gy)-w}
(FlyBase, 1992-)
Secondary FlyBase IDs
    References (2)