FB2024_03 , released June 25, 2024
Recombinant Construct: Mi{PT-GFSTF.un}
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General Information
Symbol
Mi{PT-GFSTF.un}
FlyBase ID
FBtp0072170
Feature type
transgenic_transposable_element
Component Allele(s)
Size
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Description

The Mi{PT-GFSTF.un} entry in FlyBase is used to denote the transgenic construct present in an insertion when it is known that the inserted element corresponds to one of Mi{PT-GFSTF.0}, Mi{PT-GFSTF.1} or Mi{PT-GFSTF.2}, but it is not known which of these 3 intron phase variants the inserted element corresponds to. The Mi{PT-GFSTF.un} element is generated in vivo by using phiC31:int-mediated recombination to replace the attP cassette of a Mi{MIC} element insertion with a protein trap cassette composed of a splice acceptor site, a (GGS)[[4]] flexible linker, a multiple tag cassette (EGFP-Tag:CALI(TC) ('FlAsH')-Tag:StrepII-Tag:CS(TEVp)-3xTag:FLAG), another (GGS)[[4]] flexible linker and a splice donor site.

Construct components
Component
Use(s)
Element type
Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Component Allele
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
Mi{PT-GFSTF.un}
Secondary FlyBase IDs
    References (3)