FlyBase Recombinant Construct Report: P{let7C-lacZ.12.5}

General Information

Symbol

P{let7C-lacZ.12.5}

FlyBase ID

FBtp0072028

Feature type

transgenic_transposable_element

Component Allele(s)

Ecol\lacZ^let7C.12.5

Also Known As

let-7-Cp^12.5kb::LacZ

Size

Expression Data

lacZ reporter

Associated insertion(s)

1 available

Molecular map

Description and Uses

Construct components

Component allele Ecol\lacZ^let7C.12.5

Transgenic product class

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

lacZ

reporter enzyme

Description

A 12.5kb fragment containing almost the entire first intron of lncRNA:let7C drives expression of a lacZ reporter.

(Chawla and Sokol, 2012)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

Ecol\lacZ^let7C.12.5

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

prepupal stage P1 -- pupal stage P6

neuron | subset of mushroom body

Comment: 0-24h APF

(Wu et al., 2012)

Additional Information

Statement

Reference

The number of neurons expressing EcollacZ^let-7-C.12.5 increases during pupal development. Labeling co-localizes with dac, which labels postmitotic mushroom body neurons.

(Wu et al., 2012)

Marker for

Reflects expression of

lncRNA:let7C

(Wu et al., 2012)

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

descendant(s)

Comments

Stocks (1)

Stocks containing insertion(s) of this recombinant construct

Bloomington

92822

w^*; P{let7C-lacZ.12.5}attP40; MKRS/TM6B, Tb¹

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

P{CR43344-lacZ.12.5}

(FlyBase, 1992-)

P{let-7-C-lacZ.12.5}

(FlyBase, 1996-)

P{let7C-lacZ.12.5}

(FlyBase, 1996-)

let-7-C_p^12.5kb::Lac Z

(Chawla and Sokol, 2012)

let-7-Cp^12.5::lacZ

(Chen et al., 2015)

let-7-Cp^12.5kb::LacZ

(Chawla and Sokol, 2012)

let-7-Cp^12.5kb::lacZ

(Wu et al., 2020, Marchetti and Tavosanis, 2017, Wu et al., 2012)

Secondary FlyBase IDs

References (8)

Report Sections

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