FB2024_03 , released June 25, 2024
Recombinant Construct: P{UAS-CD4-spGFP1-10}
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General Information
Symbol
P{UAS-CD4-spGFP1-10}
FlyBase ID
FBtp0070028
Feature type
transgenic_transposable_element
Component Allele(s)
Avic\GFPspGFP1-10.UAS.Tag:M(hCD4)
Also Known As
UAS-CD4::spGFP1-10, UAS-CD4-spGFP1-10, UAS-spGFP1-10, UAS-splitGFP1-10, UAS-spGFP1-10::CD4, UAS-CD4::spGFP1-10, UAS-CD4:spGFP1-10, UAS-CD4::GFP1-10, UAS-spGFP1-10
Size
Expression Data
Associated insertion(s)
2 available
Molecular map
Description and Uses
Construct components
Component allele Avic\GFPspGFP1-10.UAS.Tag:M(hCD4)
Transgenic product class
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
spGFP1-10
Tagged with
Tag:M(hCD4)
Description

UASt regulatory sequences drive expression of the spGFP1-10 split fluorescent protein fragment fused to a membrane targeting sequence (Tag:M(hCD4)). This construct is designed for use in a GRASP (GFP Reconstitution Across Synaptic Partners) assay, in which complementary split fluorescent protein fragments expressed on the extracellular membrane of different neurons can reconstitute a functional fluorescent protein at points of contact between the neurons.

(Gordon and Scott, 2009)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (4)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (24)
Reported As
Symbol Synonym
CD4::spGFP1-10
(Ting et al., 2014)
P{UAS-CD4-spGFP1-10}
(FlyBase, 1992-)
UAS- spGFP1-1-::CD4
(Karuppudurai et al., 2014)
UAS-CD4-GFP1-10
(Shearin et al., 2018)
UAS-CD4-GFP1-10
(Chen et al., 2023)
UAS-CD4-spGFP1-10
(Ho et al., 2022, Wang et al., 2022, Yang et al., 2021, King et al., 2017, Colombani et al., 2015, Mauss et al., 2015)
UAS-CD4-splitGFP1-10
(Pech et al., 2013)
UAS-CD4::GFP1-10
(Jayakumar et al., 2016, Zhang et al., 2013)
UAS-CD4::spGFP1-10
(Oliveira-Ferreira et al., 2023, He et al., 2020, Imura et al., 2020, Jung et al., 2020, Luo et al., 2020, Peng et al., 2019, Yamada et al., 2018, Omoto et al., 2017, Watanabe et al., 2017, Fushiki et al., 2016, Hückesfeld et al., 2016, Wasserman et al., 2015, Andrews et al., 2014, Galili et al., 2014, Kohsaka et al., 2014, Bader et al., 2013, Mann et al., 2013, Riemensperger et al., 2013, Silies et al., 2013, Zhou et al., 2012, Liu et al., 2010, Gordon and Scott, 2009, Gordon and Scott, 2009)
UAS-CD4::spGFP1-10
(Kurogi et al., 2023, Vallejo et al., 2015)
UAS-CD4::spGFP1-10
(Pu et al., 2018)
UAS-CD4:GFP1-10
(Roy et al., 2014)
UAS-CD4:GFP10
(Roy et al., 2014)
UAS-CD4:spGFP1-10
(Tang et al., 2017, Macpherson et al., 2015)
UAS-GFP1-10
(Cavanaugh et al., 2014)
UAS-GFP1-10
(Devineni et al., 2013)
UAS-SpGFP1-10::CD4
(Kain and Dahanukar, 2015)
UAS-cd4::spGFP1-10
(Ni et al., 2019)
UAS-spGFP1-10
(Ho et al., 2022, Doll et al., 2017, Ting et al., 2014)
UAS-spGFP1-10::CD4
(Shearin et al., 2018, Lin et al., 2016, Karuppudurai et al., 2014)
UAS-spGFP1-10
(Zhou et al., 2023, Phan et al., 2019, Lai et al., 2012)
UAS-splitGFP1-10
(Schulz et al., 2024, Vogt et al., 2016, Pech et al., 2013)
spGFP1-10::CD4
(Karuppudurai et al., 2014)
uas-mCD4::spGFP1-10
(Pitman et al., 2011)
Secondary FlyBase IDs
    References (63)