FB2024_03 , released June 25, 2024
Recombinant Construct: P{R9D11-CD4-tdTom}
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General Information
Symbol
P{R9D11-CD4-tdTom}
FlyBase ID
FBtp0068008
Feature type
transgenic_transposable_element
Component Allele(s)
Disc\RFPtdTomato.R9D11.Tag:M(hCD4)
Also Known As
R9D11-CD4-tdTomato, R9D11-tdtom
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele Disc\RFPtdTomato.R9D11.Tag:M(hCD4)
Transgenic product class
Component
Use(s)
Regulatory region(s)
GMR9D11
Encoded product / tool
tdTomato
Tagged with
Tag:M(hCD4)
Description

The "R9D11" central nervous system enhancer followed by the core promoter and 5' UTR of Hsp70 is fused upstream of the z second intron followed by sequences encoding the transmembrane domain of Hsap\CD4 fused to tdTomato. An endoplasmic reticulum export signal from Hsap\KCNJ2 is fused to the C-terminus of the tdTomato sequences. 3'UTR and polyA sequences from His2Av are present downstream of the coding region.

(Han et al., 2011)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 14 -- 17
intermediate neuronal progenitor
(Walsh and Doe, 2017)
Additional Information
Statement
Reference

At embryonic stages, DiscRFPtdTomato.R9D11.Tag:M(hCD4) is expressed in secondary neuroblasts and their progeny.

(Walsh and Doe, 2017)
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (1)
Stocks containing insertion(s) of this recombinant construct
Bloomington
35847
w1118; P{R9D11-CD4-tdTom}attP2/TM6B, Tb1
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (9)
Reported As
Symbol Synonym
9D11-tom
(Walsh and Doe, 2017)
P{R9D11-CD4-tdTom}
(FlyBase, 1992-)
R09D11-CD4-tdTomato
(Viktorin et al., 2013)
R9D11-CD4-tdTom
(Han et al., 2011)
R9D11-CD4-tdTomato
(Hakes and Brand, 2020, Hakes et al., 2018, Li et al., 2017)
R9D11-CD4::tdTomato
(Michki et al., 2021)
R9D11-tdTom
(Li et al., 2017)
R9D11-tdtom
(Plazaola-Sasieta et al., 2019)
erm-CD4-tdTomato
(Hakes and Brand, 2020)
Secondary FlyBase IDs
    References (10)