UAS regulatory sequences drive expression of pain. The full-length pain cDNA RE03641 was used a template for the pain sequence. This cDNA was found to contain a TN10 transposon following nucleotide 1795. The transposon sequence was removed using a two step PCR strategy. The PCR product was verified and found to contain a single silent mutation at nucleotide 1605 changing C into T. This PCR product was cloned into the vector and is expressed under the control of UAS regulatory sequences.