FB2024_03 , released June 25, 2024
Recombinant Construct: PBac{810.P.FSVS-2}
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General Information
Symbol
PBac{810.P.FSVS-2}
FlyBase ID
FBtp0065993
Feature type
transgenic_transposable_element
Component Allele(s)
Size
12.174
Expression Data
Associated insertion(s)
27 available
Molecular map
Description and Uses
Description

PBac{810.P.FSVS-2} is a hybrid piggyBac/P-element protein trapping construct. The protein trap sequence is composed of a splice acceptor site, a multiple tag cassette (3xTag:FLAG-Tag:StrepII-Venus-Tag:StrepII) in splice frame 2 and a splice donor site. The ATG codon of the P\T coding sequence present in this construct has been mutated, to prevent it acting as a promoter trap. The protein trap cassette is in reverse orientation relative to the PBac ends, so that when the protein trap cassette is in the forward orientation, the components of the transgene are ordered as follows: 3' PBac terminal repeat - P-element 5' end - w+mC marker - protein trap cassette - P-element 3' end - 5' PBac terminal repeat.

(Lowe et al., 2014)
Construct components
Component
Use(s)
Element type
Tagged with
Tag:FLAG
Tag:StrepII
Venus
Marker(s)
w+mC
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size

12.174

(FlyProt project., 2009.11)
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Component Allele
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
PBac{602.P.SVS-1}
(FlyProt project., 2009.11)
descendant(s)
Comments
Stocks (27)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
PBac{810.P.FSVS-2}
(FlyBase, 1996-)
PIGP810{FSVS-2}
(FlyProt project., 2009.11)
Secondary FlyBase IDs
    References (4)