FlyBase Recombinant Construct Report: P{GMR48B04-GAL4}

General Information

Symbol

P{GMR48B04-GAL4}

FlyBase ID

FBtp0060676

Feature type

transgenic_transposable_element

Component Allele(s)

Scer\GAL4^GMR48B04

Also Known As

R48B04-GAL4, R48B04

Size

Expression Data

GAL4 reporter/driver

Associated insertion(s)

1 available

Molecular map

Description and Uses

Construct components

Component

Use(s)

Marker(s)

w^+mC

Component allele Scer\GAL4^GMR48B04

Transgenic product class

Component

Use(s)

Regulatory region(s)

GMR48B04

Encoded product / tool

GAL4

binary expression system - driver

Description

A 2724 base pair fragment from the flanking non-coding or intronic region of Oamb is fused upstream of a Drosophila core synthetic promoter (DSCP) that is followed by sequence encoding a GAL4 driver.

(Pfeiffer et al., 2011.2.9)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

Scer\GAL4^GMR48B04

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

prow | faint | restricted

(Jenett and Svirskas, 2012.9.24)

adult brain | restricted

(Shyu et al., 2017, Huetteroth et al., 2015, Shuai et al., 2015, Yamagata et al., 2015, Lin et al., 2014)

mushroom body extrinsic neuron

(Shuai et al., 2015)

mushroom body gamma lobe slice 3

(Shuai et al., 2015, Yamagata et al., 2015)

mushroom body gamma lobe slice 4

(Shyu et al., 2017, Huetteroth et al., 2015, Shuai et al., 2015, Yamagata et al., 2015, Lin et al., 2014)

mushroom body gamma lobe slice 5

(Shyu et al., 2017, Huetteroth et al., 2015, Shuai et al., 2015, Yamagata et al., 2015, Lin et al., 2014)

mushroom body beta' lobe slice 2

(Shyu et al., 2017, Huetteroth et al., 2015, Shuai et al., 2015, Yamagata et al., 2015, Lin et al., 2014)

dopaminergic PAM neuron | subset

(Shyu et al., 2017, Shuai et al., 2015, Yamagata et al., 2015, Lin et al., 2014)

mushroom body gamma lobe slice 1

(Shyu et al., 2017, Huetteroth et al., 2015)

mushroom body gamma lobe slice 2

(Shyu et al., 2017, Huetteroth et al., 2015, Yamagata et al., 2015)

dopaminergic PAM neuron 1

(Shyu et al., 2017, Huetteroth et al., 2015, Lin et al., 2014)

dopaminergic PAM neuron 2

(Shyu et al., 2017, Huetteroth et al., 2015, Lin et al., 2014)

dopaminergic PAM neuron 6

(Shyu et al., 2017, Huetteroth et al., 2015, Lin et al., 2014)

dopaminergic PAM neuron 5

(Shyu et al., 2017, Huetteroth et al., 2015, Lin et al., 2014)

TH negative neuron associated with PAM cluster

(Huetteroth et al., 2015)

dopaminergic neuron | subset

(Huetteroth et al., 2015)

dopaminergic PAM neuron 8

(Shyu et al., 2017, Lin et al., 2014)

mushroom body beta lobe slice 1

(Yamagata et al., 2015)

Additional Information

Statement

Reference

ScerGAL4^GMR48B04 drives expression in a subset of dopaminergic PAM neurons, including those that project to mushroom body gamma lobe slices 1, 2, 4, 5 and mushroom body beta' lobe slice 2.

(Shyu et al., 2017)

ScerGAL4^GMR48B04 drives expression in mushroom body extrinsic neurons, including approximately 60 dopaminergic PAM neurons, that innervate mushroom body gamma lobe slices 3, 4 and 5, and mushroom body beta' lobe slice 2.

(Shuai et al., 2015)

ScerGAL4^GMR48B04 labels a subset of dopaminergic neurons including ~55 dopaminergic PAM neurons and ~12 TH negative neuron associated with PAM clusters. Collectively, these neurons innervate mushroom body gamma lobe slices 1, 2, 4 & 5 and mushroom body beta' lobe slice 2.

(Huetteroth et al., 2015)

ScerGAL4^GMR48B04 drives expression in a subset of PAM neurons that innervate mushroom body beta' lobe slice 2, beta lobe slice 1 and gamma lobe slices 1 to 5.

(Yamagata et al., 2015)

Marker for

Reflects expression of

Oamb

(Huetteroth et al., 2015, Lin et al., 2014)

Reporter construct used in assay

Images

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