A 2kb fragment of 5' spn-F promoter sequence is placed upstream of Avic\GFP coding sequences fused with spn-F coding sequences and 2kb of downstream spn-F promoter sequences.
Comment: reference states 42 hours APF
With live imaging, a spn-FGFPRA-positive protrusion can be seen at the end of the macrochaeta that was not identified in fixed tissue.