FB2024_03 , released June 25, 2024
Recombinant Construct: P{GD8354}
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General Information
Symbol
P{GD8354}
FlyBase ID
FBtp0035201
Feature type
transgenic_transposable_element
Component Allele(s)
Brd8GD8354
Size
Expression Data
Associated insertion(s)
2 available
Molecular map
Description and Uses
Construct components
Component allele Brd8GD8354
Transgenic product class
RNAi_reagent
(dos Santos, 2023.2.8)
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
dsRNA-GD8354
Description

UASt regulatory sequences drive expression of an inverted repeat.

(dos Santos, 2023.2.8)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments

Targets multiple paralogous genes.

(Dickson et al., 2007.7.18)

Expression under the control of Scer\GAL4pnr-MD237 may result in semi-lethality, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

Expression under the control of Scer\GAL4pnr-MD237 results in the absence of 0% or 50-60% of the Scer\GAL4pnr-MD237-expressing area of the notum, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

Expression of P{GD8354} under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) results in viable flies or partial lethality, depending on the particular insertion line used.

(Neely et al., 2010)

Adults expressing P{GD8354} under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) can show significantly reduced avoidance of noxious temperature (46[o]C) compared to control flies, depending on the particular insertion line used.

(Neely et al., 2010)

Expression of P{GD8354} under the control of the cardioblast-specific Scer\GAL4tin.CΔ4 driver results in viable flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4Mef2.PR results in viable flies.

(Schnorrer et al., 2010)
Stocks (1)
Stocks containing insertion(s) of this recombinant construct
VDRC
v41530
w1118; P{GD8354}v41530
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Brd8RNAi
(Flegel et al., 2016)
P{GD8354}
(Dickson et al., 2007.7.18, FlyBase, 1996-)
Secondary FlyBase IDs
    References (10)