FB2024_03 , released June 25, 2024
Recombinant Construct: P{UAS.RMCE.w+}
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General Information
Symbol
P{UAS.RMCE.w+}
FlyBase ID
FBtp0021963
Feature type
transgenic_transposable_element
Component Allele(s)
Also Known As
pUASTP2
Size
Expression Data
Associated insertion(s)
9 available
Molecular map
Description and Uses
Description

The P{UAS.RMCE.w+} element acts as a target for recombination-mediated cassette exchange (RMCE). It contains a w+mC marker flanked by a pair of inverted attP sites. The entire attP-flanked cassette can be replaced with DNA from a compatible donor plasmid (where the sequence to be inserted is flanked by inverted attB sites) through RMCE driven by the phiC31:int integrase. P{UAS.RMCE.w+} also contains UASt regulatory sequences; these are outside of the attP-flanked RMCE target cassette.

(Bateman et al., 2006)
Construct components
Component
Use(s)
Element type
Regulatory region(s)
UASt
Also carries
attP
Marker(s)
w+mC
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Component Allele
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
Comments
Stocks (5)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
P{UAS.RMCE.w+}
(FlyBase, 1996-)
P{UASTP2}
(FlyBase, 1996-)
pUASTP2
(Griffin et al., 2009, Bateman et al., 2006)
Secondary FlyBase IDs
    References (7)