construct_comment: A 4.1kb genomic fragment corresponding to the region just proximal to Hsp70Ba (nucleotides 103592-107652 of BACR28I14) has been inserted into pP{W25} using NotI and Acc65I sites. A 4.3kb genomic fragment corresponding to the region just distal to Hsp70Ba (nucleotides 109380-113666 of BACR28I14) has been inserted into pP{W25} using AscI and BsiWI sites.Donor ends-out targeting construct which is designed to result in deletion of the Hsp70Ba gene in a targeting event.
A 4.1kb genomic fragment corresponding to the region just proximal to Hsp70Ba (nucleotides 103592-107652 of BACR28I14) has been inserted into pP{W25} using NotI and Acc65I sites. A 4.3kb genomic fragment corresponding to the region just distal to Hsp70Ba (nucleotides 109380-113666 of BACR28I14) has been inserted into pP{W25} using AscI and BsiWI sites.
construct_comment: A 4.1kb genomic fragment corresponding to the region just proximal to Hsp70Ba (nucleotides 103592-107652 of BACR28I14) has been inserted into pP{W25} using NotI and Acc65I sites. A 4.3kb genomic fragment corresponding to the region just distal to Hsp70Ba (nucleotides 109380-113666 of BACR28I14) has been inserted into pP{W25} using AscI and BsiWI sites.Donor ends-out targeting construct which is designed to result in deletion of the Hsp70Ba gene in a targeting event.
A 4.1kb genomic fragment corresponding to the region just proximal to Hsp70Ba (nucleotides 103592-107652 of BACR28I14) has been inserted into pP{W25} using NotI and Acc65I sites. A 4.3kb genomic fragment corresponding to the region just distal to Hsp70Ba (nucleotides 109380-113666 of BACR28I14) has been inserted into pP{W25} using AscI and BsiWI sites.
Donor ends-out targeting construct which is designed to result in deletion of the Hsp70Ba gene in a targeting event.