construct_comment: A 3.7kb genomic fragment corresponding to the region just proximal to Hsp70Aa (nucleotides 46647-50367 of BACR48E12) has been inserted into pP{W25} using NotI and Acc65I sites. A 3.6kb genomic fragment corresponding to the region just distal to Hsp70Ab (nucleotides 56217-59857 of BACR48E12) has been inserted into pP{W25} using AscI and BsiWI sites.Donor ends-out targeting construct which is designed to result in deletion of the Hsp70 genes at 70A (Hsp70Aa and Hsp70Ab) in a targeting event.
A 3.7kb genomic fragment corresponding to the region just proximal to Hsp70Aa (nucleotides 46647-50367 of BACR48E12) has been inserted into pP{W25} using NotI and Acc65I sites. A 3.6kb genomic fragment corresponding to the region just distal to Hsp70Ab (nucleotides 56217-59857 of BACR48E12) has been inserted into pP{W25} using AscI and BsiWI sites.
construct_comment: A 3.7kb genomic fragment corresponding to the region just proximal to Hsp70Aa (nucleotides 46647-50367 of BACR48E12) has been inserted into pP{W25} using NotI and Acc65I sites. A 3.6kb genomic fragment corresponding to the region just distal to Hsp70Ab (nucleotides 56217-59857 of BACR48E12) has been inserted into pP{W25} using AscI and BsiWI sites.Donor ends-out targeting construct which is designed to result in deletion of the Hsp70 genes at 70A (Hsp70Aa and Hsp70Ab) in a targeting event.
A 3.7kb genomic fragment corresponding to the region just proximal to Hsp70Aa (nucleotides 46647-50367 of BACR48E12) has been inserted into pP{W25} using NotI and Acc65I sites. A 3.6kb genomic fragment corresponding to the region just distal to Hsp70Ab (nucleotides 56217-59857 of BACR48E12) has been inserted into pP{W25} using AscI and BsiWI sites.
Donor ends-out targeting construct which is designed to result in deletion of the Hsp70 genes at 70A (Hsp70Aa and Hsp70Ab) in a targeting event.