Transgenic construct used in an enhancer-blocking assay. Contains divergently transcribed w and eve-lacZ reporters. A 560bp fragment containing two tandem copies of the twi proximal element (2PE), a sub-fragment of the SF-1 insulator, a 1.2kb core Fab7 fragment and the 500bp eve stripe 3 enhancer (E3) have been inserted between the two reporters.
Consists of a 1231bp PstI-ApaI fragment including the Fab-7 insulator.
A 1.2kb XhoI-NotI fragment corresponding to the minimal Fab-7 boundary element.
Construct: 1.2kb Fab-7 DNA fragment that spans nucleotide positions 84,594 to 83,423 within the complete BX-C sequence and contains all three DNaseI hypersensitive sites.