An Hsp70 promoter is fused upstream of P-element sequences from nucleotides 39 to 2871 (coordinates relative to the canonical P-element described in FBrf0038996), these sequences include P\T. There are two differences with respect to the canonical P-element sequence; nucleotide 2864G (outside the P\T coding region) is deleted and the C1104T substitution is a synonymous substitution in a valine codon.
construct_comment: H{hsp/CP} produces functional P\T in both the male and female germline, but not in the soma. Heat shock treatments significantly increase transposase activity in the female germline but have little effect in the male germline. The P\T transposase activity encoded by H{hsp/CP} is repressed by the P cytotype.The H{hsp/CP}2 insertion reduces the transposase activity of P{Δ2-3}99B in the germline and in the soma.Paternally derived H{hsp/CP} transgenes do not induce gonadal dysgenesis in the offspring of M females or the offspring of P females, but do induce gonadal dysgenesis in the offspring of M\' females.