FB2024_03 , released June 25, 2024
Recombinant Construct: P{walter}
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General Information
Symbol
P{walter}
FlyBase ID
FBtp0013012
Feature type
transgenic_transposable_element
Component Allele(s)
Size
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Construct components
Component allele walter
Transgenic product class
Component
Use(s)
Encoded product / tool
Description

Construct: 2456bp of contiguous w locus sequence on one side of the site of P-element excision in "whd" allele series and 999bp on the other.

Construct: P{walLKP} contains an insertion of a 1124bp KP element (missing the first and last 15 nucleotides) compared to P{walL}. Construct: P{walL} contains an insertion of a polylinker at position -2368 (numbering scheme from FBrf0041384) compared to P{walter}. Construct: P{walLy} contains an insertion of y+t7.7 into the polylinker of P{walL}. 12 single-base changes, each of which adds or removes a restriction site relative to the canonical w gene sequence.

12 substitutions of single base pairs within a region of approximately 3kb of a w minigene.

Construct: P{walter} contains 12 single-base changes, each of which adds or removes a restriction site relative to the canonical w gene sequence. Construct: P{walLf} contains an insertion of an approximately 13,000bp f gene fragment (f+t13) into the polylinker of P{walL}. Construct: P{walLG} contains an insertion of approximately 4000bp DNA into the polylinker of P{walL}. Construct: P{walLK} contains an insertion of 1858bp DNA into the polylinker of P{walL}.

Contains a w gene with 12 single-base alterations compared to the canonical w sequence together with y gene sequences.

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
descendant(s)
Comments

construct_comment: Used to characterize mechanism of P-element transposition and repair of resulting double-strand DNA breaks.

Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Secondary FlyBase IDs
    References (8)