2.3kb of 5' DNA from Scr, exon 1, intron 1 and 100bp of exonII (including the amino terminal 3 amino acids of Scr) drives the expression of lacZ.
construct_comment: One of a number of basal reporter constructs used in studies of Scr cis-regulatory elements.
report comment: Scr-lacZ fusion gene in pP{BSRN} contains the nuclear localization signal from SV40 large T antigen; the fusion gene in pP{BSR} does not. The Scr portion of the fusion gene in the BSR constructs includes intron 1.
construct_comment: One of a number of basal reporter constructs used in studies of Scr cis-regulatory elements.
report comment: Scr-lacZ fusion gene in pP{BSRN} contains the nuclear localization signal from SV40 large T antigen; the fusion gene in pP{BSR} does not. The Scr portion of the fusion gene in the BSR constructs includes intron 1.