Gene size reduced from 34kb to 5.8 kb. Heat shock terminal sequence ligated to 3' end cDNA.
5.8kb Ace minigene consisting of 1.5kb 5' genomic DNA from the Ace region fused to a full length Ace cDNA.
Intronless Ace coding region fused to 1.5 kb of promoter sequences.
construct_comment: Progenitor(s) that of the plasmid, pP{Ace.Fb}.