Transgenic construct used to study insulator function. Contains divergently transcribed w+mC and y+t7.7 marker genes. The y sequence includes the wing and body enhancers upstream of the promoter, and the bristle enhancer in intron 1. An FRT cassette containing a Fab-7 insulator fragment and GMR regulatory sequences (5 copies of the gl binding site) have been inserted in between the y body enhancer and y promoter, at a position corresponding to 200bp proximal to the site of the gypsy element insertion found in the y2 allele.
Consists of a 1231bp PstI-ApaI fragment including the Fab-7 insulator.
A 1.2kb XhoI-NotI fragment corresponding to the minimal Fab-7 boundary element.
Construct: 1.2kb Fab-7 DNA fragment that spans nucleotide positions 84,594 to 83,423 within the complete BX-C sequence and contains all three DNaseI hypersensitive sites.