FB2024_03 , released June 25, 2024
Recombinant Construct: P{twe-lacZ}
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General Information
Symbol
P{twe-lacZ}
FlyBase ID
FBtp0009045
Feature type
transgenic_transposable_element
Component Allele(s)
twelacZ
Also Known As
twine-lacZ
Size
Expression Data
Associated insertion(s)
0 available
Molecular map
Description and Uses
Construct components
Component
Use(s)
Marker(s)
w+mW.hs
Component allele twelacZ
Transgenic product class
Component
Use(s)
Regulatory region(s)
twe
twe_twine-lacZ
Encoded product / tool
twe
Tagged with
lacZ
Description

661bp BglII fragment is excised from twe+t3.7 (leaving N-terminal 224 amino acids) and replaced with sequence encoding a lacZ reporter.

(White-Cooper et al., 1998)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter or direct label
Stage
Tissue/Position (including subcellular localization)
Reference
spermatogenesis & adult stage | male
spermatocyte
(Xu et al., 2012, Franklin-Dumont et al., 2007)
spermatid
(Franklin-Dumont et al., 2007)
Additional Information
Statement
Reference

twelacZ expression first appears faintly in nuclei of mature primary spermatocytes, becomes stronger during the meiotic divisions and is strongest in spermatids.

(Franklin-Dumont et al., 2007)
 
Marker for
Reflects expression of
twe
(Xu et al., 2012)
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
Reported As
Symbol Synonym
P{twe-lacZ}
(FlyBase, 1996-)
Twine-lacZ
(Chen et al., 2010)
twe-lacZ
(Xu et al., 2012)
twe::lacZ
(Von Stetina et al., 2008)
twine-lacZ
(Baker and Fuller, 2007, Franklin-Dumont et al., 2007, Maines and Wasserman, 1999)
Secondary FlyBase IDs
    References (8)