FB2024_03 , released June 25, 2024
Recombinant Construct: P{419}
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General Information
Symbol
P{419}
FlyBase ID
FBtp0001624
Feature type
transgenic_transposable_element
Component Allele(s)
Size
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Description

A 4.0kb fragment of sequence from the ph-p regulatory region (coordinates 1397-5329 of FBrf0054014) has been inserted into pP{CaSpeR-AUG-βgal}, in between the w+mC marker and lacZ reporter sequence. The ph-p sequence does not contain sufficient regulatory sequence to initiate a pattern of lacZ expression that reflects wild-type ph-p expression.

Construct components
Component
Use(s)
Encoded product / tool
Marker(s)
Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Component Allele
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
Comments

construct_comment: Progenitors are those of the plasmid, pP{419}.

report comment: Note: white marker is derived from w+mC from pP{CaSpeR-AUG-βgal}, however, adjacent ph sequences alter expression. Upon insertion, wph-p.419 shows an increased incidence of variegated expression patterns.

Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Secondary FlyBase IDs
    References (2)