A 2.9kb fragment of sequence from the ph-p regulatory region (coordinates 3467-6272 of FBrf0054014) has been inserted into pP{CaSpeR-AUG-βgal}, in between the w+mC marker and lacZ reporter sequence. The ph-p sequence does not contain sufficient regulatory sequence to initiate a pattern of lacZ expression that reflects wild-type ph-p expression. Variegated expression of the w+mC marker is seen in a high proportion of P{418} euchromatic insertion lines.
construct_comment: Progenitors are those of the plasmid, pP{418}.
report comment: Note: white marker is derived from w+mC from pP{CaSpeR-AUG-βgal}, however, adjacent ph sequences alter expression. Upon insertion, wph-p.418 shows an increased incidence of variegated expression patterns.