FB2024_03 , released June 25, 2024
Recombinant Construct: P{UAS-GFP::lacZ.T2}
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General Information
Symbol
P{UAS-GFP::lacZ.T2}
FlyBase ID
FBtp0001401
Feature type
transgenic_transposable_element
Component Allele(s)
Ecol\lacZUAS.T2.GFP
Size
Expression Data
Associated insertion(s)
0 available
Molecular map
Description and Uses
Construct components
Component
Use(s)
Marker(s)
w+mC
Component allele Ecol\lacZUAS.T2.GFP
Transgenic product class
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
GFP
lacZ
Description

UASt regulatory sequences drive expression of a fusion protein composed of the GFP fluorescent protein followed by a lacZ reporter.

(Timmons et al., 1997)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
pP{UAS-GFP::lacZ.T2}
(Timmons et al., 1997)
descendant(s)
Comments

construct_comment: Provides vital cell marker that can be targeted via GAL4/UAS binary system.P{UAS-GFP::lacZ.T1} differs from P{UAS-GFP::lacZ.T2} in the structure of the fusion protein (order of lacZ and GFP).

(Timmons et al., 1997)

report comment: Fusion protein has GFP at the N-terminus and βgal at the C-terminus.

(Timmons et al., 1997)
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P{UAS-GFP::lacZ.T2}
(FlyBase, 1996-)
UAST-GFP/βgal
(Timmons et al., 1997)
Secondary FlyBase IDs
    References (2)