FlyBase Recombinant Construct Report: P{Ubi-GFP.D}

General Information

Symbol

P{Ubi-GFP.D}

FlyBase ID

FBtp0001355

Feature type

transgenic_transposable_element

Component Allele(s)

Avic\GFP^Ubi-p63E.PD

Also Known As

Ubi-GFP, Ub-GFP, P{w+mC=ubi-GFP}, ubiquitin-GFP, PUbGFP

Size

Expression Data

GFP reporter

Associated insertion(s)

7 available

Molecular map

Description and Uses

Construct components

Component

Use(s)

Marker(s)

w^+mC

Component allele Avic\GFP^Ubi-p63E.PD

Transgenic product class

Component

Use(s)

Regulatory region(s)

Ubi-p63E

Encoded product / tool

GFP

green fluorescent protein

Description

1980bp of Ubi-p63E promoter (and first four amino acids) directs the expression of GFP followed by Hsp27 polyadenylation sequence.

(Davis et al., 1995)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

Avic\GFP^Ubi-p63E.PD

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

organism | ubiquitous

(Davis et al., 1995)

third instar larval stage

imaginal disc

(Roch et al., 1998)

Additional Information

Statement

Reference

In third instar larva, ubiquitous expression throughout imaginal discs. Used as marker for FRT-induced mitotic clones.

(Roch et al., 1998)

Shows bright green fluorescence in both nucleus and cytoplasm. There is a long delay between zygotic expression and appearance of fluorescence.

(Davis et al., 1995)

Marker for

Reflects expression of

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

pP{Ubi-GFP}

(Davis et al., 1995)

descendant(s)

Comments

report comment: The Ubi::GFP fusion protein is highly fluorescent when maternally supplied, but there is a long delay between zygotic expression and appearance of fluorescence.

(Davis et al., 1995)

Stocks (14)

Stocks containing insertion(s) of this recombinant construct

Bloomington

5188

w^*; P{neoFRT}82B P{Ubi-GFP.D}83

1620

w^*; P{Ubi-GFP.D}61EF P{neoFRT}80B

1681

y¹ w^67c23 P{Ubi-GFP.D}ID-1

1626

w^*; P{Ubi-GFP.D}61EF P{FRT(w^hs)}2A

5245

y¹ w^67c23 P{Ubi-GFP.D}ID-1 P{neoFRT}18A

5189

y¹ w^*; P{Ubi-GFP.D}33 P{Ubi-GFP.D}38 P{neoFRT}40A

5153

y¹ w^67c23 P{Ubi-GFP.D}ID-1 P{FRT(w^hs)}101

5154

y¹ w^67c23 P{Ubi-GFP.D}ID-1 P{FRT(w^hs)}9-2

Kyoto

108090

w^*; P{neoFRT}82B P{Ubi-GFP.D}83

106448

y¹ w^67c23 P{Ubi-GFP.D}ID-1

108116

y¹ w^67c23 P{Ubi-GFP.D}ID-1 P{neoFRT}18A/FM6

108091

y¹ w^*; P{Ubi-GFP.D}33 P{Ubi-GFP.D}38 P{neoFRT}40A/CyO

More stocks available...

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (27)

Reported As

Symbol Synonym

PUbGFP

(Brand, 1999, Davis et al., 1995)

P[Ubi-GFP]

(Parker et al., 2002)

P[UbiGFP]

(Tapon et al., 2001)

P[hs-w⁺]

(Lloyd et al., 2002)

P[hs-w[+]]

(Lloyd et al., 2002)

P[ubn-GFP]

(Spencer et al., 1998)

P{Ub::GFP w^+mC}

(Evans and Cline, 2007)

P{Ubi-GFP.D}

(Aldridge et al., 2007, FlyBase, 1996-)

P{Ubi-GFP}

(FlyBase, 1996-)

P{ubi-GFP.D}

(Letizia et al., 2019)

P{w+mC=Ubi-GFP}

(Genova and Fehon, 2003)

P{w+mC=ubi-GFP}

(Domingos et al., 2004)

UBGFP

(Suzanne et al., 2001)

Ub-GFP

(Chotard et al., 2005, Liu et al., 2003, Minami et al., 1999)

Ubi-GFP

(Ji and Tulin, 2012, Bazigou et al., 2007, Bazigou et al., 2007, Lee and Adler, 2004, Janody et al., 2003, Okada et al., 2002, Duchek and Rorth, 2001, Strutt, 2001, Geng et al., 2000)

Ubi-nls:GFP

(Cachoux et al., 2023)

Ubi::GFP

(Roper and Brown, 2004)

UbqnGFP

(Roch and Akam, 2000)

[w+mC=Ubi-GFP]

(Dobens et al., 2001)

nuclear ubiquitin-GFP

(Schulz et al., 2002)

ubi-GFP

(Ji and Tulin, 2012, Valenta et al., 2011, Dominguez et al., 2004)

ubi-GFP{w^+mC}

(Dominguez et al., 2004)

ubi-gfp

(Lee and Fischer, 2012)

ubiGFP

(Erkner et al., 2002)

ubiquitin-GFP

(Baeg et al., 2001, Bilder et al., 2000)

ubiquitinGFP

(Beccari et al., 2002)

ubiquitous nuclear GFP

(Roch et al., 2002)

Secondary FlyBase IDs

References (46)

Report Sections

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