FB2024_03 , released June 25, 2024
Recombinant Construct: P{etau-lacZ}
Open Close
General Information
Symbol
P{etau-lacZ}
FlyBase ID
FBtp0001352
Feature type
transgenic_transposable_element
Component Allele(s)
Size
12.257
Expression Data
Associated insertion(s)
64 available
Molecular map
Description and Uses
Description

P{etau-lacZ} is an enhancer trap construct that encodes a lacZ reporter that is targeted to cell processes. The enhancer trap sequence consists of the weak P\T promoter fused upstream of lacZ coding sequence that is tagged at the N-terminal end with the first 383 amino acids of Btau\MAPT (this includes sequence necessary for microtubule binding, resulting in the lacZ reporter being targeted to cell projections such as axons when expressed in neurons).

(Callahan and Thomas, 1994)
Construct components
Component
Use(s)
Element type
Encoded product / tool
lacZ
Tagged with
Tag:MT(btau)
Marker(s)
w+mC
Sequence Data
Sequence (FB)
FlyBase-compiled
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Phenotypic Data
Component Allele
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
Comments

Restriction sites useful for plasmid rescue: EcoRI and SacII (to right).

(Callahan and Thomas, 1994)
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
P[etau-lacZ]
(Callahan and Thomas, 1994)
P{etau-lacZ}
(FlyBase, 1996-)
tau-lacZ
(FlyBase, 1996-)
tau-β-gal
(FlyBase, 1996-)
Secondary FlyBase IDs
    References (8)