FlyBase Recombinant Construct Report: P{KZ.TRAP}

General Information

Symbol

P{KZ.TRAP}

FlyBase ID

FBtp0001242

Feature type

transgenic_transposable_element

Component Allele(s)

Also Known As

Kin-lacZ, Kin:βgal, kinesin-lacZ, Khc-β-gal, kin:lacZ

Size

11.983

Expression Data

Associated insertion(s)

26 available

Molecular map

Description and Uses

Description

P{KZ.TRAP} is an enhancer trap construct that encodes a lacZ reporter that can be used to trace the morphology and projections of neurons. The enhancer trap sequence is at the 5' end of the construct and consists of the weak P\T promoter fused upstream of lacZ coding sequence that is tagged at the N-terminal end with the Tag:MT(Khc) tag (this sequence targets the lacZ protein to the microtubules, and in neurons this results in the protein being targeted to cell projections such as the axon, presumably due to the tagged protein being transported along the microtubules towards their plus ends).

(Giniger et al., 1993)

Construct components

Component

Use(s)

Element type

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:MT(Khc)

microtubule protein localization tag

Marker(s)

w^+mC

Sequence Data

Sequence (FB)

FlyBase-compiled

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

11.983

Left end

P5\'

(Giniger et al., 1993)

Right end

P3\'

(Giniger et al., 1993)

Segments

Num

Orientation

Symbol

P\T::Khc::lacZ(P5',Bg2)

Reference

(Giniger et al., 1993)

Phenotypic Data

Component Allele

Phenotypic Data

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

pP{KZ.TRAP}

(Giniger et al., 1993)

descendant(s)

P{5'KZ.TRAP.MT287}

(Takasu-Ishikawa et al., 2001)

Comments

Restriction sites useful for plasmid rescue: EcoRI and SacII (to right).

(Giniger et al., 1993)

Stocks (1)

Stocks containing insertion(s) of this recombinant construct

Bloomington

8766

y¹ w^*; P{KZ.TRAP}KZ1138

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (24)

Reported As

Symbol Synonym

(Clark et al., 1997)

KZtrap

(Muralidhar et al., 1993)

Khc-β-gal

(Gervais et al., 2008)

Kin-lacZ

(Yan et al., 2011, Gervais et al., 2008, Nakamura et al., 2004)

Kin-β-gal

(Becalska and Gavis, 2010)

Kin::β-gal

(Krauss et al., 2009)

Kin:LacZ

(Jones and Macdonald, 2015)

Kin:β-Gal

(Tian and Deng, 2008)

Kin:βGal

(Coutelis and Ephrussi, 2007)

Kin:βgal

(Sanghavi et al., 2012, MacDougall et al., 2001, FlyBase, 1996-)

Kinesin::β-gal

(Mohr et al., 2001)

Kinesin:βgalactosidase

(Jankovics et al., 2001)

P{KZ.TRAP}

(FlyBase, 1996-)

P{Kin::β-Gal}

(Krauss et al., 2009)

khc-lacZ

(Becalska and Gavis, 2010)

khc-β-gal

(Motola and Neuman-Silberberg, 2004)

khc:lacZ

(Kalifa et al., 2009, FlyBase, 1996-)

kin-lacZ

(Sun et al., 2011, Januschke et al., 2006, Baum et al., 2000)

kin:lacZ

(Kalifa et al., 2009)

kin:βgal

(Kalifa et al., 2009, Han et al., 2000)

kinesin-lacZ

(Sun et al., 2011, Steinhauer and Kalderon, 2005)

kinesin-Β-gal

(Martin and St. Johnston, 2003)

kinesin-Β-galactosidase

(Martin and St. Johnston, 2003)

kinlacZ

(Han et al., 2000)

Secondary FlyBase IDs

References (38)

Report Sections

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