FlyBase Recombinant Construct Report: P{β3/lac-6.0}

General Information

Symbol

P{β3/lac-6.0}

FlyBase ID

FBtp0001220

Feature type

transgenic_transposable_element

Component Allele(s)

Ecol\lacZ^βTub60D.6.0

Size

Expression Data

lacZ reporter

Associated insertion(s)

1 available

Molecular map

Description and Uses

Construct components

Component

Use(s)

Marker(s)

w^+mW.hs

Component allele Ecol\lacZ^βTub60D.6.0

Transgenic product class

Component

Use(s)

Regulatory region(s)

betaTub60D_pw/beta3/lac-6.0

Encoded product / tool

lacZ

reporter enzyme

Description

lacZ gene expression is driven by 6kb of upstream sequences of βTub60D.

(Gasch et al., 1989)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

Ecol\lacZ^βTub60D.6.0

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 11 -- 17

somatic mesoderm

(Hinz et al., 1992)

embryonic stage 13 -- 14

visceral mesoderm

(Lord et al., 1995)

somatic mesoderm

(Lord et al., 1995)

embryonic stage 13 -- 17

somatic mesoderm

(Lord et al., 1995)

embryonic stage 16

embryonic dorsal vessel

(Kremser et al., 1999)

embryonic somatic muscle cell

(Kremser et al., 1999)

embryonic/larval somatic muscle cell

(Gasch et al., 1989)

embryonic stage 16 -- 17

pharyngeal muscle cell

(Gasch et al., 1989)

embryonic/larval somatic muscle cell

(Gasch et al., 1989)

embryonic/larval dorsal vessel

(Gasch et al., 1989)

embryonic stage 17

pharyngeal muscle cell

(Gasch et al., 1989)

embryonic/larval dorsal vessel

(Gasch et al., 1989)

Additional Information

Statement

Reference

Reporter gene expression is observed in the somatic mesoderm starting at stage 11.

(Hinz et al., 1992)

No expression in visceral muscles.

(Gasch et al., 1989)

Marker for

embryonic dorsal vessel

(Kremser et al., 1999)

Reflects expression of

βTub60D

(Kremser et al., 1999, Lord et al., 1995, Hinz et al., 1992, Gasch et al., 1989)

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

pP{β3/lac-6.0}

(Gasch et al., 1989)

descendant(s)

Comments

construct_comment: One of a series of lacZ reporter constructs used to characterize 5\' and intronic regulatory regions of βTub60D.

(Gasch et al., 1989)

Stocks (0)

Stocks containing insertion(s) of this recombinant construct

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

P{β3/lac-6.0}

(FlyBase, 1996-)

pW/β3/lac-6,0

(Kremser et al., 1999)

β3/lac-6.0

(Gasch et al., 1989)

Secondary FlyBase IDs

References (6)

Report Sections

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