FB2024_03 , released June 25, 2024
Recombinant Construct: P{UAS-CAT::GFP.nls}
Open Close
General Information
Symbol
P{UAS-CAT::GFP.nls}
FlyBase ID
FBtp0001205
Feature type
transgenic_transposable_element
Component Allele(s)
Avic\GFPUAS.CAT,Tag:NLS(SV40-largeT)
Size
Expression Data
Associated insertion(s)
0 available
Molecular map
Description and Uses
Construct components
Component
Use(s)
Marker(s)
w+mC
Component allele Avic\GFPUAS.CAT,Tag:NLS(SV40-largeT)
Transgenic product class
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
GFP
Tagged with
Tag:NLS(SV40-largeT)
Tn9CAT
Description

UASt regulatory sequences drive expression of a fusion protein comprising the Tag:NLS(SV40-largeT) nuclear localization signal, the GFP coding region and the Tn9CAT coding region (the Tn9CAT coding region has been added with a view to stabilizing GFP expression in vivo).

(Shiga et al., 1996)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
pP{UAS-CAT::GFP.nls}
(Shiga et al., 1996)
descendant(s)
Comments

report comment: Sequences for SV40 nuclear localization signal fused at 5\' end of GFP sequences.

(Shiga et al., 1996)

construct_comment: Designed as vital cell marker, however levels of fluorescence very low.

(Shiga et al., 1996)
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
GFPN-CAT
(FlyBase, 1996-)
P{UAS-CAT::GFP.N}
(FlyBase, 1996-)
P{UAS-CAT::GFP.nls}
(FlyBase, 1996-)
UAS-gfpn-CAT
(Shiga et al., 1996)
Secondary FlyBase IDs
    References (2)