FB2024_03 , released June 25, 2024
Recombinant Construct: P{ET-L}
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General Information
Symbol
P{ET-L}
FlyBase ID
FBtp0000853
Feature type
transgenic_transposable_element
Component Allele(s)
Ecol\lacZeve.ET-L
Size
Expression Data
lacZ reporter
Associated insertion(s)
2 available
Molecular map
Description and Uses
Description

P{ET-L} carries a 1.65kb fragment of the eve upstream regulatory sequences fused upstream of sequence encoding a lacZ reporter. This eve fragment includes sequence sufficient for expression of stripe 2 only of the seven-stripe eve embryonic expression pattern (FBrf0049357). Insertions of this construct can act as enhancer traps, showing specific position-dependent expression patterns in addition to the basal stripe 2 expression pattern in the embryo.

(Perrimon et al., 1991)
Construct components
Component
Use(s)
Element type
Encoded product / tool
lacZ
Marker(s)
ry+t7.2
Component allele Ecol\lacZeve.ET-L
Transgenic product class
Component
Use(s)
Encoded product / tool
Ecol\lacZ
Description

Construct: eve promoter sequence (from position -5.45kb to -4.65kb) drives the expression of Ecol\lacZ fused to a minimal Hsp70 promoter.

(Goto et al., 1989)

Ecol\lacZ expression is driven by an eve promoter.

(Perrimon et al., 1991)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
(Perrimon et al., 1991)
Additional Information
Statement
Reference

Subcellular localization: cytoplamic.

(Perrimon et al., 1991)
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
pP{ET-L}
(Goto et al., 1989)
descendant(s)
Comments

report comment: eve regulatory sequences are from the "L" element, necessary for late embryonic expression.

(Goto et al., 1989)
Stocks (1)
Stocks containing insertion(s) of this recombinant construct
Bloomington
8744
P{ET-L}trh-1-eve-1 ry506
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
ET-L
(Goto et al., 1989)
P{ET-L}
(FlyBase, 1996-)
eve-lacZ
(Perrimon et al., 1991)
Secondary FlyBase IDs
    References (3)