FB2024_03 , released June 25, 2024
Recombinant Construct: P{Icarus-neo}
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General Information
Symbol
P{Icarus-neo}
FlyBase ID
FBtp0000475
Feature type
transgenic_transposable_element
Component Allele(s)
P\Ths.PSb
Tn\neoRhs.PSb
Size
Expression Data
Associated insertion(s)
0 available
Molecular map
Description and Uses
Construct components
Component allele P\Ths.PSb
Transgenic product class
Component
Use(s)
Regulatory region(s)
Hsp70
Encoded product / tool
P\T
Description

Insertion of a 1kb fragment carrying the coding region of Tn\neoRhs.PSb between the Hsp70 promoter and the P\T open reading frames of P\Ths.PSa. Tn\neoRhs.PSb and P\Ths.PSb share the same promoter and their products are presumably produced from a polycistronic message.

(Steller and Pirrotta, 1986)
Component allele Tn\neoRhs.PSb
Transgenic product class
Component
Use(s)
Regulatory region(s)
Hsp70
Encoded product / tool
Tn\neoR
Description

1kb BglII-BamHI fragment carrying the coding region of Tn\neoR expressed from an Hsp70 promoter. The Tn\neoR coding region lies between the promoter and the coding region of P\Ths.PSb. Tn\neoRhs.PSb and P\Ths.PSb share the same promoter and their products are presumably produced from a polycistronic message.

(Steller and Pirrotta, 1986)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
pP{Icarus-neo}
(Steller and Pirrotta, 1986)
descendant(s)
Comments

report comment: P-element transposase and neoR under control of heat-shock promoter. Presumed to produce a polycistronic mRNA with independent translation initiation sites for P\T and neoR.

(Steller and Pirrotta, 1986)

construct_comment: Intervening neoR sequences in P{Icarus-neo} attenuate high levels of P\T activity observed for P{Icarus}.

(Steller and Pirrotta, 1986)
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
P{Icarus-neo}
(FlyBase, 1996-)
Secondary FlyBase IDs
    References (3)