FB2024_03 , released June 25, 2024
Recombinant Construct: P{enD}
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General Information
Symbol
P{enD}
FlyBase ID
FBtp0000315
Feature type
transgenic_transposable_element
Component Allele(s)
Ecol\lacZen.D
Size
13.204
Expression Data
lacZ reporter
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele Ecol\lacZen.D
Transgenic product class
Component
Use(s)
Encoded product / tool
lacZ
Description

Intron 1 from Dvir\en (in reverse orientation) plus a fragment of en promoter extending from -2.4kb to +188bp upstream of the transcription start site is fused upstream of lacZ.

(Kassis, 1990)
Sequence Data
Sequence (FB)
FlyBase-compiled
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size

13.204

Left end

P5\'

(Kassis, 1990)
Right end

P3\'

(Kassis, 1990)
 
Segments
Num
Orientation
Symbol
1
+
CaSpeR_P5'(P5',Sp)
2
+
001167
3
-
Dvir\en_intron1
4
+
001168
5
+
enA::lacZ(6.9)-F
6
+
001164
7
+
Car1_P3'(H3,P3')
Reference
(Kassis, 1990)
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 8 -- 12
organism | striped
(Kassis, 1990)
embryonic stage 12
neurogenic region
(Kassis, 1990)
embryonic stage 12 -- 13
fat body primordium
(Kassis, 1990)
Additional Information
Statement
Reference

In embryos, lower levels of expression observed in first 3 (anterior) stripes; in subsequent stripes, expression in even-numbered stripes is higher than that in odd-numbered stripes. Note: Results using assay for β-gal activity differ from those detecting the β-gal RNA. The stripe pattern persists into late stages of embryogenesis in the former case, but not in the latter.

(Kassis, 1990)
 
Marker for
Reflects expression of
en
(Kassis, 1990)
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
pP{enD}
(Kassis, 1990)
descendant(s)
Comments

construct_comment: Expression observed in homozygotes versus heterozygotes; tested for proximity dependence (transvection).

(Kassis et al., 1991)

construct_comment: w expressed in stripes in embryo (from the selectable marker, white mini-gene w+mC).

(Kassis, 1990)

construct_comment: One of a series of constructs used to characterize en regulatory elements contained within the first intron.

(Kassis, 1990)

construct_comment: One of a series of constructs analyzed for effects of en sequences upon expression of the adjacent w+mC gene.

(Kassis et al., 1991)

construct_comment: One of several constructs designated P{en2}, all of which contain 2.4kb of en 5\' flanking sequences and en intron 1 sequences.

(Kassis et al., 1992)
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
P{en2}
(FlyBase, 1996-)
P{enD}
(FlyBase, 1996-)
Secondary FlyBase IDs
    References (7)